P020 Differential expression of miR-424-5p and miR-378c in stenotic and penetrating lessions of Crohn’s Disease associates with altered transcription of genes involved in vascular regulation
Coll, S.(1);Seco, M.(1);Bauset, C.(1);Lis, L.(1);Cosín-Roger, J.(2);Ortiz-Masia, D.(3);Gisbert-Ferrandiz, L.(1);Barrachina, D.(1);Calatayud, S.(1);
(1)Faculty of Medicine - University of Valencia, Pharmacology, Valencia, Spain;(2)FISABIO, Unidad Mixta Facultad de Medicina-Hospital Dr Peset, Valencia, Spain;(3)Faculty of Medicine - University of Valencia, Medicine, Valencia, Spain;
Background
Crohn´s disease (CD) is often complicated by the appearance of intestinal stenosis (B2 behaviour) or fistulas (B3 behaviour), and the factors determining the pattern of disease and the etiology of these lesions, both associated with fibrosis and requiring surgery, are poorly defined. Our aim has been to detect transcriptomic alterations in intestinal tissues affected by stenosis or fistulas, and have centred our attention in genes affecting vessel formation and function as potential contributors to these lesions.
Methods
We conducted a small RNA sequencing profiling on ileal surgical resections from CD patients with B2 (n=10) or B3 (n=10) behaviour. We obtained a sample from affected tissue and the paired non-affected ileum from each patient.
Results
Fig1. Expression of pro-angiogenic miRNAs (A) and of genes associated with vascular regulation (B) in tissues from stenotic (B2) and penetrating (B3) lesions of CD patients. Data represent normalized counts of each RNA and are expressed as mean±SEM (A) or mean (B). Data in (C) correspond to Pearson’s R. Significant differences vs corresponding non-affected tissue are represented by *P<0.05 (paired t-test) and #P<0.05 (ANOVA+Tukey test)