Poster presentations: Basic Science 2020

P039 Regional distribution of TCR Vδ1 and TCR Vδ2 cells in healthy and inflamed mucosa of inflammatory bowel disease

E. Tristan, A. Carrasco, A. Martín-Cardona, Y. Zabana, M. Aceituno, D. Horta, X. Andújar, I. Salvador, C. Loras, F. Fernández-Bañares, M. Esteve

Gastroenterology Department, Hospital Universitari Mútua Terrassa, Universitat de Barcelona, Terrassa. Centro de Investigación Biomédica en red de enfermedades hepáticas y digestivas CIBERehd, Gastroenterology Department, Terrassa, Spain

Background

Vδ1⁺ and Vδ2⁺ are TCRγδ lymphocytes of the gut intraepithelial compartment that recognise proteins without help of antigen-presenting cells. Its relevance in inflammatory bowel disease (IBD) is unknown.

Methods

Aim:

To measure Vδ1⁺ and Vδ2⁺ in T-cell subtypes [CD4⁺, CD8⁺, double positive (DP,CD4⁺CD8⁺), double negative (DN,CD4⁻CD8⁻) and CD103⁺] of healthy and IBD inflammed gut mucosa.

Results

Twenty-six active IBD patients without immunosuppressants (n = 18 Crohn’s disease (CD), eight ulcerative colitis (UC)) and 10 healthy controls (paired biopsies of ileum, right and left colon) were included. Lymphocytes were analysed with LSRFortessa cytometer. Results expressed as % median (25–75%IQI).

Conclusion

Healthy mucosa: reduction in ileum of total CD3⁺Vδ1⁺ due to CD3⁺CD8⁺Vδ1⁺ and CD3⁺DN_Vδ1⁺

Healthy gut	Ileum	Right colon	Left colon	p-value
CD3⁺Vδ1⁺	2,6 (1.2–5.7)	11.0 (7.9–16.1)	7.1 (5.1–9.6)	0.001
CD3⁺CD8⁺Vδ1⁺	2.7 (1.3–5.6)	13.4 (7.4–18.1)	10.6 (6.3–15.6)	0.005
CD3⁺DN_Vδ1⁺	29.8 (17.8–39.4)	55.5 (37.7–72.4)	51.2 (45.5–67.7)	0.003

Ileal CD vs. ileal control: CD3⁺DN_Vδ2⁺ reduction and increase of Vδ1 and Vδ2 CD4⁺.

Ileum	CD	Healthy	p-value
CD3⁺DN_Vδ2⁺	5.5 (1.9–9.7)	15.9 (9.9–36)	0.021
CD3⁺Vδ2⁺DN	44.9 (27.1–56.9)	69.60 (52.9–73.5)	0.027
CD3⁺Vδ1⁺CD4⁺	6.1 (2.7–16.8)	1.8 (0.5–4.0)	0.043
CD3⁺Vδ2⁺CD4⁺	20.8 (6.6–45.3)	2.5 (0–5.6)	0.003

Colonic CD, UC vs. control: CD3⁺Vδ1⁺ decrease in CD and UC, and decrease of their subsets CD3⁺CD8⁺Vδ1⁺ and CD3⁺DN_Vδ1⁺. CD103⁺ and CD103⁻ showed specular behaviour with CD3⁺DN_Vδ1⁺CD103⁺ and CD3⁺CD8⁺Vδ1⁺CD103⁺ decrease and CD3⁺DN_Vδ1⁺CD103⁻ and CD3⁺CD8⁺Vδ1⁺CD103⁻ increase both in UC and CD. A similar effect was observed for CD103⁺ and CD103⁻ of CD3⁺DN_Vδ2⁺. CD3⁺Vδ1⁺CD4⁺ was increased in UC and ileal CD.

	UC	CD	Healthy	p-value
CD3⁺Vδ1⁺	2.1 (1.6–3.0)	2.8 (1.9–20.6)	7.1 (5.0–9.6)	0.002
CD3⁺CD8⁺Vδ1⁺	2.3 (1.1–5.8)	3.7 (2.8–23.7)	10.6 (6.4–15.6)	0.013
CD3⁺DN_Vδ1	18.9 (16.2–27.5)	28.4 (20.5–61.2)	51.2 (45.5–67.8)	0.001
CD3⁺CD8⁺_Vδ1⁺CD103⁺	49.9 (17.2–60.9)	45.0 (13.3–84.5)	93.8 (87.7–98.4)	0.002
CD3⁺CD8⁺Vδ1⁺CD103⁻	50.0 (32.1–82.8)	55.0 (15.5–86.7)	6.1 (1.6–12.3)	0.003
CD3⁺DN_Vδ1⁺CD103⁺	7.4 (2.9–14.9)	48.5 (16.4–82.3)	91.3 (76.3–96.1)	0.001
CD3⁺DN_Vδ1⁺CD103⁻	92.5 (85.1–97.1)	51.5 (17.7–83.6)	8.7 (3.8–23.6)	0.001
CD3⁺DN_Vδ2⁺CD103⁺	6.5 (0.9–8.8)	4.2 (0–30.0)	59.8 (18.5–67.2)	0.011
CD3⁺DN_Vδ2⁺CD103⁻	93.5 (91.2–99.03)	72.2 (33.8–97.9)	37.5 (27.5–52.2)	0.004
CD3⁺Vδ1⁺CD4⁺	4.6 (2.2–15.3)	1.4 (0.7–5.8)	0.6 (0.3–1.5)	0.006

Reduction of Vδ1+ and Vδ2+, mainly of CD103+, may play a role in IBD pathophysiology by perpetuating inflammation. Increase of CD3+Vδ1+CD4+ in both Ileal CD and UC may compensate this decrease with a selective increase in ‘helper’ function.

Posted in: Poster presentations: Basic Science 2020

ECCO'24 Abstracts

P039 Regional distribution of TCR Vδ1 and TCR Vδ2 cells in healthy and inflamed mucosa of inflammatory bowel disease

Background

Methods

Results

Conclusion